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Detection of Extended-Spectrum B-Lactamase (ESBL) Production in Clinical Isolates of Escherichia Coli Recovered From Patients in Tertiary Care Hospital of Gujarat

Author:Vipul M Khakhkhar, Pragnesh J Bhuva, Shashwati P Bhuva, Chirag P Patel, Jaydev M Pandya, Meera S Cholera

Keywords:ESBL, multi-drug resistant, MIC reduction test.

Type:Original Article

Abstract:Background: The presence of Extended-Spectrum ?-lactamase (ESBL) producing organisms significantly affects the course and outcome of an infection and poses a challenge to infection management worldwide. Hence, the knowledge about their prevalence is important to guide towards appropriate antibiotic treatment. Aims & Objective: The purpose of this study is to determine the prevalence and susceptibility of ESBL in Escherichia Coli isolated from different clinical samples. Methods: A total of 100 isolates of E. Coli were collected over a period of six months. Antimicrobial susceptibility testing was determined by commonly used antibiotics using Kirby-Bauer’s disc diffusion method. ESBL detection was done by the screening method and then confirmed by the phenotypic confirmatory test with combination disc and the minimum inhibitory concentration (MIC) reduction test as recommended by the (CLSI). Results: Out of 100 E. coli isolates, 69 were positive by screening test and 58 were potential ESBL producers by combination disc and MIC reduction test. Multidrug resistance was significantly (p < 0.01) higher in ESBL positive isolates. Conclusion: Knowledge of the prevalence of ESBL and resistance pattern of bacterial isolates in a geographical area will help the clinicians to formulate the guidelines for antibiotic therapy.